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Chemically modified biomacromolecules—i. They are extensively used not only to elucidate cellular processes but also in industrial applications, particularly in the context of biopharmaceuticals.
This splitting of spectra may be due to transitions of electrons in biomacromolecules from the electronic term S1 to excited vibrational levels of the ground state S0. In this case, a population inversion at the transitions S1S0 can be implemented if the population of the S1 term is sufcient.
Let us estimate the intensity I0 of laser radiation that is necessary for inversion superuorescence under conditions of the experiments performed.
Normalized spectra of two-photon excited luminescence TPEL of dashed line dimedrol, 1 DNAdimedrol mixture and 2 nucleohistondimedrol mixture. Dynamics of the increase of TPEL of the nucleohistondimedrol mixture in time: spectra are measured 1 at the beginning of the experiment, 2 30 min, and 3 50 min after the beginning of the experiment.
Dynamics of the quenching of TPEL of the DNA dimedrol mixture in time: spectra are measured 1 at the beginning of the experiment, 2 30 min, and 3 50 min after the beginning of the experiment. The laser beam was focused into a spot 23 mm in diameter at the sample. This laser was rather efcient for the excitation of TPEL in the studies of electronvibrational spectra of proteins, DNA, nucleohiston, and their components purines, pyrimidines, and amino acids [3, 4]. Both experimental studies and the corresponding theoretical estimates allow us to assume rather condently that luminescence enhancement in genostructures is implemented in vitro under the applied regimes of two-photon excitation with the use of dimedrol as an activator.
In other words, the emission of DNA and nucleohiston is of a superuorescent type. It is not improbable that endogenic compounds that directly or indirectly interact with DNA and chromo-. The conditions when in vivo electronic population inversion may occur in genostructures, similar to the conditions implemented in the regimes of TPEL, are not clear.
These conditions can be achieved in biosystems, for example, due to photonphonon interactions in DNA within the framework of the Dicke theory [5]. However, such interactions are related to purely physical mechanisms. As for the physicobiochemical processes resulting in laser pumping of DNA and chromosomes in vivo, we can predict P. Garyaev the existence of high-power ATP systems in biosystems that deliver energy for the transition of gene structures into Frlich biocoherent states, similar to the states that were obtained as a particular case in our work.
This work was supported by the Russian Foundation for Basic Research, project no. Garyaev, P. Blagodatskikh, V. Agaltsov, A. Li, K. Singapore: World Scientic , p. Open navigation menu. Close suggestions Search Search. User Settings. Skip carousel. Carousel Previous.
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